Columbia, Mo. Missouri scored three goals in each half to hand Kansas University its worst loss since the 1999 season, a 6-0 Tigers victory Friday at Walton Soccer Field.
The Tigers improved to 13-5 overall and 7-3 in the Big 12, while KU fell to 11-7-1 and 4-5-1.
Kansas surrendered a goal in the ninth minute to Alysha Bonnick, who headed in a cross from Mo Redmond at 8:49.
Missouri scored a second goal on a similar play in the 33rd minute as Michelle Collins headed in a Kat Tarr cross deep in the box.
The Tigers scored four times over the next 21 minutes to hand KU its first loss in Columbia since the 2002 season. It was also MU's first victory over the Jayhawks in the last seven meetings.
KU will head to San Antonio, Texas, for the Big 12 Championships, which begins Wednesday. Seventh-seeded Kansas will face second-seeded Texas A&M at 7:30 p.m. Wednesday.