Kabul, Afghanistan ? In a stark warning to U.S. forces, the Afghan government said it will try to regulate the presence of U.S. troops and their use of airstrikes, while the U.N. on Tuesday announced that “convincing evidence” exists that an American-led operation killed 90 civilians.

The U.N. sent in a team of investigators, who relied solely on villagers’ statements in alleging the American-led operation in the western province of Herat on Friday killed 60 children and 30 adults. The U.S. military stood by its account, that 25 militants and five civilians were killed in the operation.

“I don’t have any information that would suggest that our military commanders in Afghanistan don’t believe, still, that this was a legitimate strike on a Taliban target,” Defense Department spokesman Bryan Whitman said in Washington.

The U.N. allegation comes a day after President Hamid Karzai’s government said it will try to put more controls on the way American and NATO troops operate, a response to a series of airstrikes and other operations this summer that have caused the deaths of scores of civilians.

Afghanistan’s Council of Ministers ordered the ministries of defense and foreign affairs to open negotiations with the U.S. and NATO over the use of airstrikes, house searches and the detentions of Afghan civilians. It also called for a “status of force” agreement to regulate the troops’ presence.

Afghanistan’s effort to rein in foreign forces is similar to steps taken by the Iraqi government, which has demanded a timetable for the withdrawal of U.S. troops and greater control of U.S. operations until their departure.

The U.N.’s allegation of such a large number of civilian deaths could set the U.S., U.N. and the Afghan government on a collision course over the use of military force in Afghan villages, where international troops battle Taliban and al-Qaida militants daily.

Russia on Tuesday circulated a draft Security Council press statement expressing serious concern about the numerous civilian casualties reportedly caused by the airstrike and saying member nations “strongly deplore the fact that this is not the first incident of this kind.”

Press statements must be approved by all 15 Security Council members and Western diplomats said that there was no chance the Russian draft would be adopted.

The draft, obtained by The Associated Press, recognizes the need to combat terrorism, but notes “that killing and maiming of civilians is a flagrant violation of international humanitarian law and human rights law.”

It calls on the U.S.-led coalition, the International Security Assistance Force and all parties in Afghanistan to take steps to ensure the protection of civilians, particularly women and children.

The Russians called for an investigation of the incident.

A recent spate of civilian deaths has added fuel to long-simmering public anger surrounding the issue. In the first week of July, 69 Afghan civilians were killed in two separate operations in eastern Afghanistan, including 47 people killed in Nangarhar province while walking to a wedding party, Afghan officials say.

Afghan officials say that scores of civilians – between 76 and 90 – were killed in Herat province on Friday. The head of the Afghan Independent Human Rights Commission, Ahmad Nader Nadery, has confirmed reports that a memorial ceremony was being held for a militia commander allied with the Afghan police and several relatives and friends from outside the area were staying overnight in the village at the time of the attack.

Civilian casualties have long been a major source of friction between Karzai and his Western backers. Afghan officials say civilian deaths create a rift between the government and the people that Taliban and other anti-government forces use as leverage to turn villagers away from the government.

According to an Associated Press tally, 705 civilians have been killed this year: 536 by militants, and 158 by international forces; 11 civilians have died in cross fire. The numbers do not include figures from the Herat battle and likely do not account for all civilian deaths this year.

U.S. and NATO officials say they take great care in their targeting but also accuse the militants of hiding in civilian homes and using Afghans as human shields.