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A New Use for Rum...
Well I have been busy what with the end of the semester and the holidays but here is a link to a little recipe some of you might want to try...extracting DNA using rum. This idea comes from a really cool site called instructables.com. The materials are all everyday items: * 1/4 of a shot glass full of your saliva * several drops of dish soap (look for sodium laurel sulfate in the ingredients) * a pinch of table salt (1/16 of a teaspoon) * some contact-lens cleaning solution, meat tenderizer, or pineapple juice (optional) * Ice-cold 120-proof+ liquor (overproof rum works well)
The saliva provides the DNA, The dish soap is to break open the cell membranes, the salt and the rum is to keep the DNA from staying in solution, the contact lens cleaning solution or meat tenderizer or pineapple juice contain stable forms of enzymes called proteases that digest the proteins in the cells containing the DNA.The extraction process, detailed here is really easy but I am not sure I would do the last step...tasting the DNA. This procedure is basically the same one used in labs to isolate DNA it's just that we use fancier sounding chemicals that have been purified and standardized. Also what you end up with is both DNA and RNA, but hey this is still pretty exciting even if you don't want to taste the DNA.If you don't want to use saliva, a good source of DNA is dried peas. You could puree maybe a quarter cup of dried peas with an equal amount of water in a blender, use a quarter shot glass of that. Use the rest of the recipe as given. You should be able to get a better yield of DNA and RNA than with the saliva.Play this video to see the the DNA extraction process:
5 minute DNA Extraction in a Shot Glass - More DIY How To Projects